Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 6, Pages 1101-1106Publisher
SPRINGER
DOI: 10.1007/s10529-008-9654-0
Keywords
co-expression; cytochrome P450; Streptomyces peuceticus; whole-cell biotransformation
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A biotransformation system was designed to co-express CYP107P3 (CSP4), cytochrome P450, from Streptomyces peuceticus, along with CamA (putidaredoxin reductase) and CamB (putidaredoxin) from Pseudomonas putida, the necessary reducing equivalents, in a class I type electron-transfer system in E. coli BL21 (DE3). This was carried out using two plasmids with different selection markers and compatible origins of replication. The study results showed that this biotransformation system was able to mediate the O-dealkylation of 7-ethoxycumarin.
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