Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 12, Pages 2097-2103Publisher
SPRINGER
DOI: 10.1007/s10529-008-9821-3
Keywords
Betaine; E. coli; Ethanol; Lactate; Osmotic stress
Categories
Funding
- U. S. Department of Agriculture [01-35504-10669, 00-52104-9704]
- U. S. Department of Energy [FG02-96ER20222, FG36-04GO14019]
- Verenium Corporation
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A lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly inserting a promoterless mini-Tn5 cassette (transpososome) containing the complete Zymomonas mobilis ethanol pathway (pdc, adhA, and adhB) into the chromosome. By selecting for fermentative growth in mineral salts medium containing xylose, a highly productive strain was isolated in which the ethanol cassette had been integrated behind the rrlE promoter, designated strain LY160 (KO11, Delta frd::cekY(EC) Delta adhE Delta ldhA, Delta ackA lacA::casAB(Ko) rrlE::(pdc(Zm)-adhA(Zm)-adhB(Zm)-FRT-rrlE) pflB(+)). This strain fermented 9% (w/v) xylose to 4% (w/v) ethanol in 48 h in mineral salts medium, nearly equal to the performance of KO11 with Luria broth.
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