Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 12, Pages 2091-2096Publisher
SPRINGER
DOI: 10.1007/s10529-008-9817-z
Keywords
Decolorization; Pichia methanolica; Purification; Recombinant laccase; Remazol Brilliant Blue R
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Funding
- Tianjin Natural Sciences Foundation of China [07JCZDJC03100]
- Tianjin Municipal Science and Technology Commission
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A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l(-1) after 16 h at 45 degrees C and pH 5 when 25 U laccase ml(-1) was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators.
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