Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 11, Pages 1893-1899Publisher
SPRINGER
DOI: 10.1007/s10529-008-9786-2
Keywords
cytotoxicity; gene expression profile; mouse liver; nanoparticle; silver
Categories
Funding
- KOSEF [M10203000039- 05M0300-03910]
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Mice were fed either 13 nm silver nanoparticles or 2-3.5 mu m silver microparticles. The livers were then obtained after 3 days and subjected to a histopathological analysis. The nanoparticle-fed and microparticle-fed livers both exhibited lymphocyte infiltration in the histopathological analysis, suggesting the induction of inflammation. In vitro, a human hepatoma cell line (Huh-7) was treated with the same silver nanoparticles and microparticles. The mitochondrial activity and glutathione production were hardly affected. However, the DNA contents decreased 15% in the nanoparticle-treated cells and 10% in the microparticle-treated cell, suggesting a more potent induction of apoptosis by the nanoparticles. From a microarray analysis of the RNA from the livers of the nano- and micro-particle-fed mice, the expression of genes related to apoptosis and inflammation was found to be altered. These gene expression changes in the nanoparticle-treated livers lead to phenotypical changes, reflecting increased apoptosis and inflammation. The changes in the gene expression were confirmed by using a semi-quantitative RT-PCR.
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