Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 10, Pages 1839-1845Publisher
SPRINGER
DOI: 10.1007/s10529-008-9759-5
Keywords
Alzheimer's disease; edible vaccine; immunogenicity; tomato-derived beta amyloid
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Funding
- Ministry of Education and Science of the Korean government
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Human beta-amyloid (A beta) is believed to be one of the main components of Alzheimer's disease, so reduction of A beta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for A beta with tandem repeats. Integration of the human A beta gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the A beta protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human A beta antigen.
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