Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 9, Pages 1621-1626Publisher
SPRINGER
DOI: 10.1007/s10529-008-9735-0
Keywords
global regulatory gene; pikromycin; S-adenosylmethionine synthetase; Streptomyces venezuelae; transcription
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Funding
- Republic of Korea
- Korea Research Foundation (KRF)
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Two regulator genes, metK1-sp and afsR-sp, from Streptomyces peucetius ATCC 27952 were heterologously expressed in S. venezuelae ATCC 15439, to produce 14-membered pikromycin antibiotics. The production of pikromycin was increased by 1.6-fold and 2.6-fold by the expression of metK1-sp and afsR-sp, respectively. The overexpression of metK1-sp and afsR-sp in S. venezuelae stimulated the expression of the pathway-specific regulatory genes, pikD and ketosynthase, as demonstrated by RT-PCR. The elevated transcripts of the pikD and ketosynthase genes were consistent with the enhanced production of pikromycin.
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