Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 9, Pages 1671-1677Publisher
SPRINGER
DOI: 10.1007/s10529-008-9736-z
Keywords
co-culture; Linum album; Piriformospora indica; plant/fungi interactions; podophyllotoxin; Sebacina vermifera
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Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and 1.9 mg/l, respectively after 12 d cultivation. Co-cultures of L. album cells with axenically cultivable arbuscular mycorrhiza-like fungi, Piriformospora indica and Sebacina vermifera, were established for the first time. These enhanced podophyllotoxin and 6-methoxypodophyllotoxin production by about four- and eight-fold, respectively, along with a 20% increase in biomass compared to the control cultures.
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