Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1002, Issue -, Pages 260-266Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2015.08.018
Keywords
Metabolomics; Lipidomics; Sample preparation; LC-MS
Funding
- CTSI NIH [UL1 TR000064]
- SECIM NIH [U24 DK097209]
- UF Chemistry Department
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The goal of this research was to find the most comprehensive lipid extraction of blood plasma, while also providing adequate aqueous preparation for metabolite analysis. Comparisons have been made previously of the Folch, Bligh-Dyer, and Matyash lipid extractions; furthermore, this paper provides an additional comparison of a phospholipid removal plate for analysis. This plate was used for lipid extraction rather than its intended use in lipid removal for polar analysis, and it proves to be robust for targeted lipid analysis. Folch and Matyash provided reproducible recovery over a range of lipid classes, however the Matyash aqueous layer compared well to a typical methanol preparation for polar metabolite analysis. Thus, the Matyash method is the best choice for an untargeted biphasic extraction for metabolomics and lipidomics in blood plasma. (C) 2015 Elsevier B.V. All rights reserved.
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