Journal
BIOTECHNOLOGY JOURNAL
Volume 14, Issue 3, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201800220
Keywords
carbon flux; chrysolaminarin; lipid biosynthesis; metabolic rewiring; microalga
Funding
- Natural Science Foundation of Guangdong Province [2014A030308010]
- Natural Science Foundation of China [41576132]
- China Postdoctoral Science Foundation [2017M612838]
- Open Project Program of Guangxi Colleges and Universities Key Laboratory of Utilization of Microbialand Botanical Resources [1130001006400020003]
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Chrysolaminarin, the primary polysaccharide reservoir in some marine algae, has attracted much attention due to its broad health properties. However, its biosynthetic pathway and regulation mechanisms have rarely been reported which hinders the improvement of production efficiency. Therefore, this study aims to identify key metabolic nodes in the chrysolaminarin biosynthetic pathway. A phosphoglucomutase (PGM) in the model microalga Phaeodactylum tricornutum, revealing its critical role in chrysolaminarin biosynthesis is identified. PGM overexpression significantly elevates chrysolaminarin content by 2.54-fold and reaches 25.6% of cell dry weight; while algal growth and photosynthesis are not impaired. Besides, PGM overexpression up- and down-regulates the expression of chrysolaminarin and lipid biosynthetic genes, respectively. Microscopic analysis of aniline blue stained cells reveals that overproduced chrysolaminarin localized predominantly in vacuoles. Lipidomic analyses reveal that PGM overexpression significantly reduces the lipid content. The findings reveal the critical role of PGM in regulating the carbon flux between carbohydrate and lipid biosynthesis in microalgae, and provide a promising candidate for high efficiency production of chrysolaminarin.
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