4.5 Review

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

HaloTag-based purification of functional human kinases from mammalian cells

Rachel Friedman Ohana et al.

PROTEIN EXPRESSION AND PURIFICATION (2011)

Article Biochemical Research Methods

The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal

James M. Vergis et al.

PROTEIN EXPRESSION AND PURIFICATION (2011)

Article Biochemical Research Methods

Application of protein engineering to enhance crystallizability and improve crystal properties

Zygmunt S. Derewenda

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2010)

Article Biotechnology & Applied Microbiology

High quality protein microarray using in situ protein purification

Keehwan Kwon et al.

BMC BIOTECHNOLOGY (2009)

Article Biochemical Research Methods

Use of proteomics for design of a tailored host cell for highly efficient protein purification

Zhu Liu et al.

JOURNAL OF CHROMATOGRAPHY A (2009)

Article Biochemical Research Methods

Refolding and purification of histidine-tagged protein by artificial chaperone-assisted metal affinity chromatography

Xiao-Yan Dong et al.

JOURNAL OF CHROMATOGRAPHY A (2009)

Article Biochemical Research Methods

A Carboxy-Terminal Affinity Tag for the Purification and Mass Spectrometric Characterization of Integral Membrane Proteins

Julie P. Wong et al.

JOURNAL OF PROTEOME RESEARCH (2009)

Review Biochemistry & Molecular Biology

Making the Most of Fusion Tags Technology in Structural Characterization of Membrane Proteins

Hao Xie et al.

MOLECULAR BIOTECHNOLOGY (2009)

Article Biochemical Research Methods

Bacterial expression of a eukaryotic membrane protein in fusion to various Mistic orthologs

Hay Dvir et al.

PROTEIN EXPRESSION AND PURIFICATION (2009)

Article Biochemical Research Methods

High-throughput T7 LIC vector for introducing C-terminal poly-histidine tags with variable lengths without extra sequences

Jonas Lee et al.

PROTEIN EXPRESSION AND PURIFICATION (2009)

Article Biochemical Research Methods

A simple and efficient expression and purification system using two newly constructed vectors

Huanting Liu et al.

PROTEIN EXPRESSION AND PURIFICATION (2009)

Article Biochemistry & Molecular Biology

Crystallization of the first three domains of the human insulin-like growth factor-1 receptor

Neil M. Mckern et al.

PROTEIN SCIENCE (2009)

Article Biochemistry & Molecular Biology

Mistic: Cellular localization, solution behavior, polymerization, and fibril formation

Hay Dvir et al.

PROTEIN SCIENCE (2009)

Article Biochemistry & Molecular Biology

HatoTag: A novel protein labeling technology for cell imaging and protein analysis

Georgyi V. Los et al.

ACS CHEMICAL BIOLOGY (2008)

Review Biochemical Research Methods

Epitope tagging

Bill Brizzard

BIOTECHNIQUES (2008)

Article Biochemical Research Methods

Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions

Li Liu et al.

PROTEIN EXPRESSION AND PURIFICATION (2008)

Article Biochemistry & Molecular Biology

Thioredoxin as a fusion tag for carrier-driven crystallization

Lorenzo Corsini et al.

PROTEIN SCIENCE (2008)

Article Biochemistry & Molecular Biology

Enhanced protein expression in mammalian cells using engineered SUMO fusions:: Secreted phospholipase A2

Raymond J. Peroutka et al.

PROTEIN SCIENCE (2008)

Article Biochemical Research Methods

A combined approach to improving large-scale production of tobacco etch virus protease

Paul G. Blommel et al.

PROTEIN EXPRESSION AND PURIFICATION (2007)

Article Biochemistry & Molecular Biology

Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase

John E. Dominy et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2007)

Article Multidisciplinary Sciences

High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae

Simon Newstead et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Article Biochemical Research Methods

Systematic analysis of fusion and affinity tags using human aspartyl-tRNA synthetase expressed in E-coli

Catherine M. Guzzo et al.

PROTEIN EXPRESSION AND PURIFICATION (2007)

Article Biotechnology & Applied Microbiology

Fusion to a pull-down domain:: A novel approach of producing Trigonopsis variabilis D-amino acid oxidase as insoluble enzyme aggregates

Jozef Nahalka et al.

BIOTECHNOLOGY AND BIOENGINEERING (2007)

Article Multidisciplinary Sciences

SUMOylation regulates kainate-receptor-mediated synaptic transmission

Stephane Martin et al.

NATURE (2007)

Article Multidisciplinary Sciences

Modification of nuclear PML protein by SUMO-1 regulates Fas-induced apoptosis in rheumatoid arthritis synovial fibroblasts

Ingmar Meinecke et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Article Cell Biology

PIASy mediates NEMO sumoylation and NF-κB activation in response to genotoxic stress

Angela M. Mabb et al.

NATURE CELL BIOLOGY (2006)

Article Biotechnology & Applied Microbiology

Assessment of prokaryotic collagen-like sequences derived from streptococcal Scl1 and Scl2 proteins as a source of recombinant GXY polymers

Runlin Han et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2006)

Review Biochemical Research Methods

Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

Jos Arnau et al.

PROTEIN EXPRESSION AND PURIFICATION (2006)

Article Biophysics

Characterization of the family of Mistic homologues

Tarmo P. Roosild et al.

BMC STRUCTURAL BIOLOGY (2006)

Article Biochemical Research Methods

High-throughput protein purification using an automated set-up for high-yield affinity chromatography

J Steen et al.

PROTEIN EXPRESSION AND PURIFICATION (2006)

Article Biochemical Research Methods

Enhanced soluble protein expression using two new fusion tags

DK Chatterjee et al.

PROTEIN EXPRESSION AND PURIFICATION (2006)

Article Biochemistry & Molecular Biology

Polyarginine as a multifunctional fusion tag

SM Fuchs et al.

PROTEIN SCIENCE (2005)

Article Biochemical Research Methods

Expression and purification of SARS coronavirus proteins using SUMO-fusions

X Zuo et al.

PROTEIN EXPRESSION AND PURIFICATION (2005)

Review Biotechnology & Applied Microbiology

Making the most of affinity tags

DS Waugh

TRENDS IN BIOTECHNOLOGY (2005)

Article Biochemical Research Methods

Comparison of affinity tags for protein purification

JJ Lichty et al.

PROTEIN EXPRESSION AND PURIFICATION (2005)

Article Biochemistry & Molecular Biology

Sumoylation silences the plasma membrane leak K+ channel K2P1

S Rajan et al.

Article Biochemistry & Molecular Biology

The 1.8-Å crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands

DA Breustedt et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2005)

Review Biochemical Research Methods

SUMO fusion technology for difficult-to-express proteins

TR Butt et al.

PROTEIN EXPRESSION AND PURIFICATION (2005)

Article Biochemical Research Methods

An epitope tag derived from human transcription factor IIB that reacts with a polyol-responsive monoclonal antibody

SJ Duellman et al.

PROTEIN EXPRESSION AND PURIFICATION (2004)

Article Biochemical Research Methods

Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast

J Graumann et al.

MOLECULAR & CELLULAR PROTEOMICS (2004)

Article Multidisciplinary Sciences

Heterodimerization of type II phytochromes in Arabidopsis

RA Sharrock et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

Review Biochemical Research Methods

In vitro protein refolding by chromatographic procedures

M Li et al.

PROTEIN EXPRESSION AND PURIFICATION (2004)

Article Biochemistry & Molecular Biology

Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture

IP Korndörfer et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2004)

Article Multidisciplinary Sciences

Global analysis of protein expression in yeast

S Ghaemmaghami et al.

NATURE (2003)

Review Biochemical Research Methods

A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa

RJ Jenny et al.

PROTEIN EXPRESSION AND PURIFICATION (2003)

Review Biotechnology & Applied Microbiology

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

K Terpe

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2003)

Article Biotechnology & Applied Microbiology

High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris

M Boettner et al.

JOURNAL OF BIOTECHNOLOGY (2002)

Review Biochemical Research Methods

Advances in gentle immunoaffinity chromatography

RR Burgess et al.

CURRENT OPINION IN BIOTECHNOLOGY (2002)

Article Biochemistry & Molecular Biology

AMP-activated protein kinase kinase:: detection with recombinant AMPK α1 subunit

SR Hamilton et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2002)

Article Biochemical Research Methods

Expression and purification of homogenous proteins in Saccharomyces cerevisiae based on ubiquitin-FLAG fusion

A Einhauer et al.

PROTEIN EXPRESSION AND PURIFICATION (2002)

Article Biochemical Research Methods

One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-tag

AD Keefe et al.

PROTEIN EXPRESSION AND PURIFICATION (2001)

Article Biochemistry & Molecular Biology

Control of intrinsic transcription termination by N and NusA: The basic mechanisms

I Gusarov et al.

Review Biochemical Research Methods

Twenty-five years of immobilized metal ion affinity chromatography: past, present and future

GS Chaga

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS (2001)

Article Biochemical Research Methods

A novel purification method for histidine-tagged proteins containing a thrombin cleavage site

MH Hefti et al.

ANALYTICAL BIOCHEMISTRY (2001)

Article Multidisciplinary Sciences

The use of mRNA display to select high-affinity protein-binding peptides

DS Wilson et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)

Article Biochemistry & Molecular Biology

Design of high-throughput methods of protein production for structural biology

RC Stevens

STRUCTURE (2000)

Article Multidisciplinary Sciences

Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose

G Carrard et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)

Article Biochemistry & Molecular Biology

Expression and purification of recombinant proteins by fusion to maltose-binding protein

P Riggs

MOLECULAR BIOTECHNOLOGY (2000)