Journal
BIOTECHNOLOGY JOURNAL
Volume 7, Issue 12, Pages 1509-1516Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201200220
Keywords
Chromatography; Endotoxin; Pre-Clinical; Protein production; Purification
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The removal of endotoxin from protein solutions and its prevention are key to the success of recombinant protein production due to the possible pyogenic response in mammals caused by contaminated samples. In the pre-clinical situation, protein production is often carried out in a non-good manufacturing practice (GMP) setting, utilizing bacterial DNA for transient transfection and non-validated cleaning techniques. Here, we present our findings evaluating various options for endotoxin removal, and propose strategies for endotoxin prevention with emphasis on chromatographic separations, endotoxin-removing membranes and on-column wash strategies.
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