Journal
ANALYTICAL METHODS
Volume 10, Issue 44, Pages 5313-5320Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8ay01260a
Keywords
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Funding
- Tianjin Municipal Science and Technology Commission [16PTSYJC00130]
- National Key R&D Program of China [2016YFD0401204]
- National Natural Science Foundation of China [31501566]
- International Science and Technology Cooperation Program of China [2014DFR30350]
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We have proposed a sensitive fluorescence immunoassay for detecting bisphenol A (BPA) in barreled drinking water, bottled mineral water, and river water using the anti-BPA antibody conjugated carboxyl-functionalized NaYF4:Yb/Tm upconversion nanoparticles (UCNPs) (emission maximum at 454 nm with excitation at 980 nm) as the signal probe and the coating antigen conjugated carboxyl-functionalized magnetic polystyrene microspheres (MPMs) as the capture probe. The proposed assay has a linear detection range of 0.1 to 500 g L-1 (R-2 = 0.9954). The water samples without any pretreatment can be directly analyzed. The limit of detection (LOD) of BPA in the water samples is 0.02 g L-1. The recoveries of BPA from spiked water samples for the proposed assay range from 85.35% to 108.35%. Low concentrations of BPA have been detected in the real barreled drinking water and river water samples, and the results are validated by high-performance liquid chromatography (HPLC) to be reliable, reflecting the good practicability of the proposed assay. The proposed fluorescence immunoassay can serve as a useful detection approach for the simple, rapid, sensitive, and accurate determination of BPA in drinking and environmental water.
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