Journal
BIOTECHNOLOGY JOURNAL
Volume 6, Issue 2, Pages 150-155Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201000224
Keywords
Hydrodynamic cell trapping; Microfluidics; Pseudo-nitzschia; Ultrasonic cell lysis
Funding
- Center for Embedded Networked Sensing (CENS) through the National Science Foundation of U.S.A. [CCR-0120778]
- China National Funds for Distinguished Young Scientists [60725102]
- National Natural Science Foundation of China [31000448]
- China Postdoctoral Science Foundation [20100471737]
- China Scholarship Council
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We present a microfluidic platform, which provides a simple and efficient means for handling and processing Pseudo-nitzschia, a neurotoxin-producing marine algae. Currently, analyzing the production of such toxins is complicated by multiple environmental factors and high variability among individual Pseudo-nitzschia species. To address this issue, we developed a device that can precisely trap single and multiple cells for subsequent lysis to extract relevant intracellular molecules. Our results show a cell trapping efficiency of up to 96%, which is achieved by hydrodynamic flow focusing. Additionally, complete cell lysis via ultrasonication can be accomplished within a few seconds. This platform can be applied to other algae and non-algae cell types with minimal modification, thus providing a valuable tool for studying biological intracellular mechanisms at the single and multi-cell level.
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