Journal
BIOTECHNOLOGY JOURNAL
Volume 5, Issue 5, Pages 511-514Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.200900297
Keywords
Biochips; Ion channel; Lipid bilayer; Nanobiotechnology; Pin tool
Funding
- National Research Foundation of Korea [2009-0068624]
- NSF CAREER [0644442]
- NIH [1R43GM088890-01]
- Librede, Inc.
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0644442] Funding Source: National Science Foundation
- National Research Foundation of Korea [2009-0068624] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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As a step towards an automated and operator-free ion channel measurement platform we have previously demonstrated a solution formulation for artificial lipid bilayers that enabled the indefinite storage and shipping of frozen bilayer precursors. In this work, the solutions were deposited by hand. Here, we have adapted pin tools to deposit the bilayer precursor solutions onto multi-element arrays, a popular method for microarray solution deposition. The pin tools have enabled the deposited volume to be applied highly repeatably and controllably, resulting in reduction of bilayer formation times to <1 h. The pin tools are also compatible with computerized motion control platforms, enabling automated and high throughput production. We discuss these results and the prospects of this technology to produce high density bilayer arrays for high throughput measurement of ion channels incorporated into artificial bilayers.
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