Journal
ADVANCED SYNTHESIS & CATALYSIS
Volume 360, Issue 4, Pages 696-703Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.201701029
Keywords
Enzyme catalysis; Oxygenation; Carboxylic acids; Biotransformation; Fatty acids
Categories
Funding
- National Research Foundation of Korea (NRF) - Korea government (MEST) [2017008670]
- Marine Biomaterials Research Center grant from Marine Biotechnology Program - Ministry of Oceans and Fisheries, Republic of Korea [D11013214H480000100]
Ask authors/readers for more resources
Regiospecific oxyfunctionalization of renewable long chain fatty acids into industrially relevant C9 carboxylic acids has been investigated. One example was biocatalytic transformation of 10,12-dihydroxyoctadecanoic acid, which was produced from ricinoleic acid ((9Z,12R)-12-hydroxyoctadec-9-enoic acid) by a fatty acid double bond hydratase, into (R)-3-hydroxynonanoic acid, 9-hydroxynonanoic acid, and 1,9-nonanedioic acid with a high conversion yield of ca. 70%. The biotransformation was driven by enzyme/whole-cell biocatalysts, consisting of the esterase of Pseudomonas fluorescens and the recombinant Escherichia coli expressing the secondary alcohol dehydrogenase of Micrococcus luteus, the Baeyer-Villiger monooxygenase of Pseudomonas putida KT2440 and the primary alcohol/aldehyde dehydrogenases of Acinetobacter sp. NCIMB9871. The high conversion yields and the high product formation rates over 20 U/g dry cells with insoluble reactants indicated that various (poly-hydroxy) fatty acids could be converted into multi-functional products via the simultaneous enzyme/whole-cell biotransformations. This study will contribute to the enzyme-based functionalization of hydrophobic substances.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available