Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 18, Issue 6, Pages 1062-1066Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-013-0282-8
Keywords
Escherichia coli; gamma-aminobutyric acid; glutamate decarboxylase; Neurospora crassa
Categories
Funding
- Next-Generation BioGreen 21 Program (SSAC), Rural Development Administration, Republic of Korea [PJ00954904]
Ask authors/readers for more resources
This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30 degrees C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available