Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 17, Issue 5, Pages 1041-1047Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-012-0213-0
Keywords
Bacillus licheniformis; bioflocculant; molasses; sugarcane industry
Categories
Funding
- Ministry of Science and Technology of the People's Republic of China [2009EG111023]
- Fujian Development and Reform Commission [[2010]983]
- Shenzhen Key Laboratory of Microbial Genetic Engineering
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A bioflocculant produced by B. licheniformis was investigated with regard to a low-cost culture medium and its industrial application. Molasses replaced sucrose as the sole carbon source in bioflocculant fermentation. The optimum low-cost culture medium was determined to be composed of 20 g/L molasses, 0.4 g/L urea, 0.4 g/L NaCl, 0.2 g/L KH2PO4, 1.6 g/L K2HPO4, and 0.2 g/L MgSO4. The bioflocculant from B. licheniformis was then applied to treat sugarcane-neutralizing juice to remove colloids, suspended particles, and coloring matters in a sugar refinery factory. The optimal operation conditions were a bioflocculant dosage of 21 U/mL, pH 7.3 and a heating temperature of 100A degrees C. The color and turbidity of the sugarcane juice reached IU 1267 and IU 206, respectively, after clarification with the bioflocculant; these values were almost the same as those acquired following treatment with polyacrylamide (PAM), the most widely applied flocculant in sugar industries. These results suggest the great potential for use of bioflocculants in the sugar refinery process.
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