Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 16, Issue 4, Pages 698-705Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-011-0081-z
Keywords
Haematococcus lacustris; astaxanthin; cDNA microarray; transcriptomics; high irradiance; nutrient starvation
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Funding
- Ministry of Education, Science and Technology, Republic of Korea [MG08-0305-1-0]
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In this study, a cDNA microarray was developed from over 60,000 mRNA readings to analyze the expression profiles of transcriptomes of Haematococcus lacustris under astaxanthin-inducing culture conditions, high irradiance and nitrate starvation. Among 20,033 genes on the cDNA microarray, 2,675 genes exhibited a twofold or greater difference in expression. Of these, 1,333 genes were up-regulated and 1,342 genes were down-regulated. A significant decrease in the expression of chlorophyll biosynthesis and light harvesting complex (LHC) related genes were observed under astaxanthin inducing conditions (forming red cyst cells). On the other hand, respiration-related genes, lipid metabolism-related genes and stress response-related genes were activated in the red cyst cells under stress conditions. These results enabled a better understanding of the cell responses during stress induction of H. lacustris such as photosynthesis, respiration, and some biopathways.
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