Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 993, Issue -, Pages 26-35Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2015.04.040
Keywords
Column-switching; Organic-inorganic hybrid monolith; Liquid chromatography-tandem mass; spectrometry; Plasma samples; Schizophrenia
Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/10705-9]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
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This study reports on the development of a rapid, selective, and sensitive column-switching liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to analyze sixteen drugs (antidepressants, anticonvulsants, anxiolytics, and antipsychotics) in plasma samples from schizophrenic patients. The developed organic-inorganic hybrid monolithic column with cyanopropyl groups was used for the first dimension of the column-switching arrangement. This arrangement enabled online pre-concentration of the drugs (monolithic column) and their subsequent analytical separation on an XSelect SCH C-18 column. The drugs were detected on a triple quadrupole tandem mass spectrometer (multiple reactions monitoring mode) with an electrospray ionization source in the positive ion mode. The developed method afforded adequate linearity for the sixteen target drugs; the coefficients of determination (R-2) lay above 0.9932, the interassay precision had coefficients of variation lower than 6.5%, and the relative standard error values of the accuracy ranged from -14.0 to 11.8%. The lower limits of quantification in plasma samples ranged from 63 to 1250 pg mL(-1). The developed method successfully analyzed the target drugs in plasma samples from schizophrenic patients for therapeutic drug monitoring (TDM). (C) 2015 Elsevier B.V. All rights reserved.
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