Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 15, Issue 2, Pages 285-292Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-009-0131-y
Keywords
Torulopsis glabrata; osmotic stress; proline; compatible solute; organic acid
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Funding
- National Outstanding Youth Foundation of China [20625619]
- Fok Ying-Tong Education Foundation [111075]
- Foundation for the Author of National Excellent Doctoral Dissertation of China [200962]
- Major State Basic Research Development Program of China (973 Program) [2007CB714303]
- National High-Tech Research Development Plan of China (863) [2006AA02Z201]
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This study investigated that the importing of compatible solute proline could enhance the growth of the yeast Torulopsis glabrata under hyperosmotic stress. Osmolarity progressively increased from 860 to 2,603 mOsmol/kg by accumulation of sodium pyruvate in the culture broth, leading to a significant decrease in cell growth. When 1.0 g/L of proline as a compatible solute was added to the culture medium, it was imported and enhanced cell growth by 59.0% at 2,603 mOsmol/kg. By addition of proline during pyruvate production, the concentration, productivity, and yield of pyruvate increased 22.1, 38.4, and 14.3%, respectively. These results suggested that T glabrata can import proline as an osmo-protectant against high osmotic stress, thus enhance pyruvate productivity. The improvement of yeast growth and viability under hyperosmotic stress by the addition of proline provided an alternative approach to enhance the organic acids production by yeast.
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