Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 14, Issue 2, Pages 175-179Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-008-0126-0
Keywords
genome shuffling; orthogonal matrix method; protoplast fusion; pristinamycin; Streptomyces pristinaespiralis
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Funding
- National Natural Science Foundation of China [20576122]
- Department of Science and Technology, Zhejiang Province, China [2004C13007]
- Natural Science Foundation of Zhejiang Province, China [Y404291]
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To isolate an improved pristinamycin producing strain of Streptomyces pristinaespiralis, the technique of Genome shuffling was used which resulted in a high-yield recombinant G 3-56 strain. Strain G 3-56 yielded 322 +/- 17 mg/L of pristinamycin which was 11.4-fold higher than that of the initial strain and 3.7-fold higher than strain UN-78 which previously had the highest yield of pristinamycin. The genetic characteristics of the recombinant G 3-56 strain was stable as revealed by our subculture experiments. The optimal production medium was determined using the orthogonal matrix method. Under the optimal medium conditions, the maximum yield of pristinamycin was 412 mg/L with about 1.24-fold higher than the original medium.
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