Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 14, Issue 6, Pages 748-755Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-009-0053-8
Keywords
strain screening; xylanase extraction; solid-state fermentation; drying; sugarcane bagasse; soybean meal
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Xylanases are glycosidases mainly responsible for the hydrolysis of beta-1,4 linkages in xylan. Xylanase was produced in this work by solid-state fermentation using agro industrial residues with Aspergillus niger strain, which was screened through qualitative and quantitative methods. Extraction processes with different solvents were evaluated. Solvent volume, time, and agitation speed (shaker) were optimized using statistical designs. Drying studies of the solid fermented material were also conducted in a laboratory oven where the following conditions were applied: 42 degrees C and 50 degrees C for 20 h and 80 degrees C for 1 h; 50 degrees C and 75 degrees C for 6 and 3 h, respectively. Best extraction conditions were 37 mL of solvent composed by NaCl solution (0.9%) with Tween 80 (0.1%) in 3 g of cultured material with agitation at 133 rpm in shaker for 4 min. Highest xylanase activity was 2,327 IU/gdm. The drying at 42 degrees C for 20 h provided a better maintenance of xylanase activity (58% of xylanase activity). (C) KSBB
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