Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 13, Issue 6, Pages 683-689Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-008-0055-y
Keywords
PAMAM dendrimer; DNA chip; fluorescence; avidin-biotin conjugation
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Funding
- Korea Foundation for International Cooperation of Science AMP
- Technology (KICOS) [K20601000002-07E0100-00230]
- Korean Government [KRF-2006-005-J03603]
- Seoul Research and Business Development Program [10920]
- Ministry of Education, Science & Technology (MoST), Republic of Korea [2007-00476] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity. (C) KSBB
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