Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 985, Issue -, Pages 38-47Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2015.01.014
Keywords
Extrahepatic metabolism; Human intestinal microflora system; Curcumin; UPLC-Q-TOF MS; MetaboLynx(XS); Metabolite
Funding
- National Natural Science Foundation of China [81102500, 81230080]
- Zhejiang Medicine and Health Platform Backbone Personnel Fund Scheme [2013RCA015]
- Department of Science and Technology for Analysis Test [2014C37059]
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Curcumin, a yellow pigment derived from the rhizomes of Curcuma longa Linn, is a natural antioxidant that exhibits a variety of pharmacological activities and therapeutic properties. However, as curcumin is generally conjugated when absorbed through the intestine, free curcumin is present at extremely low levels in the body. Thus, curcumin metabolites are presumed to be responsible for curcumin bioactivity. In this study, we describe a strategy using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) with automated data analysis software (MetaboLynx(XS)) for rapid analysis of the metabolic profile of curcumin in human intestinal flora. The results show that curcumin undergoes extensive phase land phase II metabolism. A total of 23 curcumin metabolites were detected and identified in vitro. Furthermore, we identified a number of novel metabolic pathways of curcumin in the human intestinal microflora system. (C) 2015 Elsevier B.V. All rights reserved.
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