4.6 Article

Rapid Determination of Cellulose

Journal

BIOTECHNOLOGY AND BIOENGINEERING
Volume 111, Issue 11, Pages 2355-2357

Publisher

WILEY-BLACKWELL
DOI: 10.1002/bit.25276

Keywords

cellulose; Updegraff assay; acid hydrolysis; biomass compositional analysis

Funding

  1. Energy Biosciences Institute

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The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff. Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%). Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method. (C) 2014 Wiley Periodicals, Inc.

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