Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 112, Issue 4, Pages 827-831Publisher
WILEY
DOI: 10.1002/bit.25482
Keywords
single cell oil; Zygomycetes; non-aseptic conditions; essential oils; bioreactor; two-stage production process
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Funding
- project Aristeia
- European Union (European Social Fund)
- Greek State (Ministry of Education and Religious Affairs - Greek General Secretariat for Research and Technology)
- Greek General Secretariat for Research and Technology
- German Federal Ministry for Education and Research
- European Union
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The use of plant oils as feedstock for the biodiesel manufacture has many drawbacks, thus, the interest has turned to single cell oil (SCO) as an alternative. However, the production of SCO is still too expensive, mainly due to the low oil productivity and the high cost of medium sterilization required. In this work raw glycerol was converted into SCO by oleaginous Zygomycetes under non-aseptic conditions on selective (i.e., containing essential oils and/or antibiotics) nitrogen limited media. The obtained data showed that although bacterial populations inhibited the fungal growth, lipid accumulation remained unaffected by the presence of bacteria in the growth medium compared to control experiments (conducted under aseptic conditions). Therefore, a two-stage process was developed in which growth was performed under aseptic conditions (1st stage) followed by lipid accumulation performed under non-aseptic conditions (2nd stage) in the presence of thyme essential oil as an antibacterial agent. Large amounts of lipids were accumulated inside the mycelia, yielding around 13% wt/wt of oil per glycerol consumed. Biotechnol. Bioeng. 2015;112: 827-831. (c) 2014 Wiley Periodicals, Inc.
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