Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 111, Issue 5, Pages 876-884Publisher
WILEY
DOI: 10.1002/bit.25148
Keywords
biodesulfurization; dibenzothiophene; Rhodococcus erythropolis IGTS8; rate-limiting step; power input per volume; aggregation
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Funding
- National Institutes of Health (NIH) [T32GM008334]
- Saudi Aramco
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A mechanistic analysis of the various mass transport and kinetic steps in the microbial desulfurization of dibenzothiophene (DBT) by Rhodococcus erythropolis IGTS8 in a model biphasic (oil-water), small-scale system was performed. The biocatalyst was distributed into three populations, free cells in the aqueous phase, cell aggregates and oil-adhered cells, and the fraction of cells in each population was measured. The power input per volume (P/V) and the impeller tip speed (v(tip)) were identified as key operating parameters in determining whether the system is mass transport controlled or kinetically controlled. Oil-water DBT mass transport was found to not be limiting under the conditions tested. Experimental results at both the 100mL and 4L (bioreactor) scales suggest that agitation leading to P/V greater than 10,000W/m(3) and/or v(tip) greater than 0.67m/s is sufficient to overcome the major mass transport limitation in the system, which was the diffusion of DBT within the biocatalyst aggregates. Biotechnol. Biotechnol. Bioeng. 2014;111: 876-884. (c) 2013 Wiley Periodicals, Inc.
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