Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 111, Issue 1, Pages 1-15Publisher
WILEY
DOI: 10.1002/bit.25096
Keywords
protein engineering; recombinase; genome engineering
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Funding
- National Institutes of Health [DP1CA174426]
- National Institute of General Medicine Sciences fellowship [T32GM080209]
- NATIONAL CANCER INSTITUTE [DP1CA174426] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM080209] Funding Source: NIH RePORTER
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Site-specific recombinases are tremendously valuable tools for basic research and genetic engineering. By promoting high-fidelity DNA modifications, site-specific recombination systems have empowered researchers with unprecedented control over diverse biological functions, enabling countless insights into cellular structure and function. The rigid target specificities of many sites-specific recombinases, however, have limited their adoption in fields that require highly flexible recognition abilities. As a result, intense effort has been directed toward altering the properties of site-specific recombination systems by protein engineering. Here, we review key developments in the rational design and directed molecular evolution of site-specific recombinases, highlighting the numerous applications of these enzymes across diverse fields of study. Biotechnol. Bioeng. 2014;111: 1-15. (c) 2013 Wiley Periodicals, Inc.
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