Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 109, Issue 11, Pages 2932-2940Publisher
WILEY
DOI: 10.1002/bit.24562
Keywords
cell printing; piezoelectric; inkjet; drug screening; tissue engineering; clogging
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The sedimentation and aggregation of cells within inkjet printing systems has been hypothesized to negatively impact printer performance. The purpose of this study was to investigate this influence through the use of neutral buoyancy. Ficoll PM400 was used to create neutrally buoyant MCF-7 breast cancer cell suspensions, which were ejected using a piezoelectric drop-on-demand inkjet printing system. It was found that using a neutrally buoyant suspension greatly increased the reproducibility of consistent cell counts, and eliminated nozzle clogging. Moreover, the use of Ficoll PM400 was shown to not affect cellular viability. This is the first demonstration of such scale and accuracy achieved using a piezoelectric inkjet printing system for cellular dispensing. Biotechnol. Bioeng. 2012; 109: 29322940. (c) 2012 Wiley Periodicals, Inc.
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