4.6 Article

Beyond growth rate 0.6: Corynebacterium glutamicum cultivated in highly diluted environments

Journal

BIOTECHNOLOGY AND BIOENGINEERING
Volume 110, Issue 1, Pages 220-228

Publisher

WILEY-BLACKWELL
DOI: 10.1002/bit.24616

Keywords

growth rate; C; glutamicum; microfluidics; single cell; acetate

Funding

  1. Bundesministerium fur Bildung und Forschung (BMBF) [0316017B]

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Fast growth of industrial microorganisms, such as Corynebacterium glutamicum, is a direct amplifier for the productivity of any growth coupled or decoupled production process. Recently, it has been shown that C. glutamicum when grown in a novel picoliter bioreactor (PLBR) exhibits a 50% higher growth rate compared to a 1?L batch cultivation [Grunberger et al. (2012) Lab Chip]. We here compare growth of C. glutamicum with glucose as substrate at different scales covering batch cultivations in the liter range down to single cell cultivations in the picoliter range. The maximum growth rate of standard batch cultures as estimated from different biomass quantification methods is mu = 0.42 +/- 0.03\ h-1 even for microtiter scale cultivations. In contrast, growth in a microfluidic perfusion system enabling analysis of single cells reproducibly reveals a higher growth rate of mu = 0.62 perpendicular to 0.02 h- 1. When in the same perfusion system cell-free supernatant from exponentially grown shake flask cultures is used the growth rate of single cells is reduced to mu = 0.47 +/- 0.02 h-1. Likewise, when fresh medium is additionally supplied with 5?mM acetate, a growth rate of mu = 0.51 perpendicular to 0.01 h-1 is determined. These results prove that higher growth rates of C. glutamicum than known from typical batch cultivations are possible, and that growth is definitely impaired by very low concentrations of byproducts such as acetate. Biotechnol. Bioeng. 2013; 110: 220228. (c) 2012 Wiley Periodicals, Inc.

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