Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 108, Issue 10, Pages 2371-2379Publisher
WILEY
DOI: 10.1002/bit.23191
Keywords
aptamer; downstream processing; magnetic beads
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Funding
- German Research Foundation (DFG)
- German Federation of Industrial Research Associations [KF 0479002 KMD3]
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Aptamers are synthetic nucleic acid-based high affinity ligands that are able to capture their corresponding target via molecular recognition. Here, aptamer-based affinity purification for His-tagged proteins was developed. Two different aptamers directed against the His-tag were immobilized on magnetic beads covalently. The resulting aptamer-modified magnetic beads were characterized and successfully applied for purification of different His-tagged proteins from complex E. coli cell lysates. Purification effects comparable to conventional immobilized metal affinity chromatography were achieved in one single purification step. Moreover, we have investigated the possibility to regenerate and reuse the aptamer-modified magnetic beads and have shown their long-term stability over a period of 6 months. Biotechnol. Bioeng. 2011; 108: 2371-2379. (C) 2011 Wiley Periodicals, Inc.
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