Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 107, Issue 2, Pages 195-205Publisher
WILEY
DOI: 10.1002/bit.22838
Keywords
glycoside hydrolases; catalytic mechanism; catalytic base; nucleophile; diversity
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Funding
- DOE Office of Biological and Environmental Research
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Recent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/nucleophile. A variety of mechanisms are used to replace this function, including substrate-assisted catalysis, a network of several residues, and the use of non-carboxylate residues or exogenous nucleophiles. Removal of the catalytic base/nucleophile by mutation can have a profound impact on substrate specificity, producing enzymes with completely new functions. Biotechnol. Bioeng. 2010;107: 195-205. (c) 2010 Wiley Periodicals, Inc.
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