Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 108, Issue 4, Pages 742-749Publisher
WILEY
DOI: 10.1002/bit.22989
Keywords
butanol-tolerance; heat resistance; artificial transcription factor; butanol production
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Funding
- Ministry of Education, Science and Technology of Korea [MG08-0204-1-0, M10748222314-08N4800 31410]
- Korea Science and Engineering Foundation [R01-2008-000-20559-0]
- Korean Ministry of Knowledge Economy [N02071165]
- National Research Foundation of Korea [R01-2008-000-20559-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli. In particular, E. coli growth is severely inhibited by butanol, being almost completely stopped by 1% (vol/vol) butanol. Here we developed a new method to increase the butanol-tolerance of E. coli with artificial transcription factor (ATF) libraries which consist of zinc finger (ZF) DNA-binding proteins and an E. coli cyclic AMP receptor protein (CRP). Using these ATFs, we selected a butanol-tolerant E. coli which can tolerate up to 1.5% (vol/vol) butanol, with a concomitant increase in heat resistance. We also identified genes of E. coli that are associated with the butanol-tolerance. These results show that E. coli can be engineered as a promising host for high-yield butanol production. Biotechnol. Bioeng. 2011; 108: 742-749. (C) 2010 Wiley Periodicals, Inc.
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