Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 106, Issue 4, Pages 553-563Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/bit.22724
Keywords
photoautotrophic biomass production; rate-limiting factors, inorganic carbon limitation, phosphorus limitation; light irradiance limitation
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Funding
- British Petroleum (BP)
- Science Foundation of Arizona (SFAz)
- Korea Research Foundation [KRF-2007-357-D00149]
- National Research Foundation of Korea [2007-357-D00149] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Microbial photosynthesis presents a valuable opportunity to capture abundant light energy to produce renewable bioenergy and biomaterials To understand the factors that control the productivity of photosynthetic microorganisms, we conducted a series of semi-continuous experiments using bench-scale photobioreactor (PM) systems, the cyanobacterium Syncchocystis PCC6803 ( PCC6803), and light conditions imitating actual day night light irradiance (1,1) Our results demonstrate that using normal BG-11 medium resulted in severe phosphate (P,) limitation for continuous operation Mitigation of P(1)-limitation, by augmenting the P, content of BG-11, allowed higher biomass productivity; however, once P(1)-limitation was alleviated, limitation by inorganic carbon (C(1)) or LI occurred. C(1)-limitation was detected by a low total C, concentration (<5 mg C/L) and high and fluctuating pH. C(1)-limitation was relieved by delivering more CO(2), which led to a stable pH in the range of 7-9 and at least 5 mg/L of C(1) in HCO(3)(-). LI limitation, evidenced by an average LI <14 W/m(2) for PCC6803, was induced by a high biomass concentration of 1,300 mg/L Thus, this work provides quantitative tools of stoichiometry and kinetics to evaluate limitation on PBRs. Biotechnol, Bioeng. 2010,106 553-563. (C) 2010 Wiley Periodicals, Inc.
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