Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 103, Issue 5, Pages 944-955Publisher
WILEY
DOI: 10.1002/bit.22330
Keywords
E. coli; astaxanthin; cyanobacteria; metabolic engineering; beta-carotene ketolase; beta-carotene hydroxylase
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Funding
- UK's Engineering and Physical Sciences Council (EPSRC) [EP/E036252/1, GR/S84347/01]
- The University of Sheffield
- Ocean Nutrition Canada
- EPSRC [EP/E036252/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [GR/S84347/01, EP/E036252/1] Funding Source: researchfish
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Carotenoid biosynthesis is highly conserved and well characterized up to the synthesis of beta-carotene. Conversely, the synthesis of astaxanthin from beta-carotene is less well characterized. Regardless, astaxanthin is a highly sought natural product, due to its various industrial applications and elevated antioxidant capacity. In this article, 12 beta-carotene ketolase and 4 beta-carotene hydroxylase genes, isolated from 5 cyanobacterial species, are investigated for their function, and potential for microbial astaxanthin synthesis. Further, this in vivo comparison identifies and applies the most promising genetic elements within a dual expression vector, which is maintained in Escherichia coli. Here, combined overexpression of individual beta-carotene ketolase and beta-carotene hydroxylase genes, within a beta-carotene accumulating host, enables a 23.5-fold improvement in total carotenoid yield (1.99 mg g(-1)), over the parental strain, with >90% astaxanthin. Biotechnol. Bioeng. 2009;103: 944-955. (C) 2009 Wiley Periodicals, Inc.
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