Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 101, Issue 4, Pages 761-767Publisher
WILEY
DOI: 10.1002/bit.21949
Keywords
transketolase; triosphosphate isomerase D-xylulose 5-phosphate; operating windows
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Funding
- Biotechnology and Biological Sciences Research Council Funding Source: Medline
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In this article we report on the enzymatic synthesis of D-xylulose 5-phosphate using triosephosphate isomerase and transketolase. Two potential starting substrates are possible with this scheme. The data presented here allow a comparison of both routes for the synthesis, based on experimental information on reaction kinetics. Operational guidelines are proposed which should assist in the scale-up of such syntheses.
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