Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 62, Issue 1, Pages 24-31Publisher
WILEY
DOI: 10.1002/bab.1236
Keywords
Salvia miltiorrhiza; transgenic hairy roots; tanshinones; elicitors; metabolic engineering; biosynthesis
Funding
- National Natural Science Fund [31270007, 31201261, 30900110]
- Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China [131041]
- New Century Talent Project [NECT-13-0902]
- Shanghai Talent Development Fund
- Open Funding Project of the State Key Laboratory of Bioreactor Engineering
- Shanghai Science and Technology Committee Project [10JC1412000, 09QH1401900]
- Shanghai Education Committee Fund [13ZZ104, 09ZZ138, J50401]
- Key Science and Technology Project of Yantze River Delta [10140702018]
- Shanghai Normal University [SK201230, SK201236]
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Tanshinone is a group of active diterpenes, which are widely used in the treatment of cardiovascular disease. In this study, methyl jasmonate (MJ) and salicylic acid (SA) were used to investigate their effects on tanshinone accumulation and biosynthetic gene expression in the hairy roots of geranylgeranyl diphosphate synthase (SmGGPPS) overexpression line (G50) in Salvia miltiorrhiza. High-performance liquid chromatography analysis showed that total tanshinone content in G50 was obviously increased by 3.10-fold (11.33mg/g) with MJ at 36H and 1.63 times (5.95mg/g) after SA treatment for 36H in comparison with their mimic treatment control. Furthermore, quantitative reverse-transcription PCR analysis showed that the expression of isopentenyl-diphosphate delta-isomerase (SmIPPI), SmGGPPS, copalyl diphosphate synthase (SmCPS), and kaurene synthase-like (SmKSL) increased significantly with MJ treatment. However, the expression of SmIPPI reached the highest level at 144H, whereas those of SmGGPPS, SmCPS, and SmKSL only increased slightly with SA treatment. The two elicitor treatments suggested that tanshinone accumulation positively correlated to the expression of key genes such as SmGGPPS, SmCPS, and SmKSL. Meanwhile, the study also indicated that it was a feasible strategy to combine elicitor treatment with transgenic technology for the enhancement of tanshinone, which paved the way for further metabolic engineering of tanshinone biosynthesis.
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