Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1384, Issue -, Pages 88-96Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2015.01.050
Keywords
Boronic acid; Monolithic column; Glycoprotein; Enrichment; Binding strength; Synergistic multiple binding
Funding
- National Science Fund for Distinguished Young Scholars [21425520]
- National Natural Science Foundation of China [21275073]
- Key Grant of 973 Program from the Ministry of Science and Technology of China [2013CB911202]
- Shanghai Tobacco Group Co., Ltd., China [00551]
Ask authors/readers for more resources
Boronate affinity materials, as effective sample enrichment sorbents for glycoproteomic analysis, have attracted increasing attention in recent years. However, most of boronate affinity materials suffer from an apparent limitation, limited binding strength. As a result, extraction of glycoproteins of trace concentration is rather difficult or impossible. In this study, we present a high boronate avidity monolithic capillary. Branched polyethyleneimine (PEI) was used as a scaffold to amplify the number of boronic acid moieties. While 2,4-difluoro-3-formyl-phenylboronic acid (DFFPBA), which exhibited ultrahigh affinity toward cis-diol-containing compounds, was employed as an affinity ligand. Due to the PEI-assisted synergistic multivalent binding, the monolithic column exhibited high boronate avidity toward glycoproteins, with binding constants of 10(-6)-10(-7) M. Such binding strength was the highest among already reported boronic acid-functionalized materials that can be used for glycoproteomic analysis. Besides, the boronate avidity monolithic column exhibited one additional beneficial feature, lowered binding (>= 6.5). These features greatly favored the selective enrichment of trace glycoproteins from real samples. The feasibility for practical applications was demonstrated with the selective enrichment of trace glycoproteins in human saliva. As compared with other boronate avidity/affinity materials, the boronate avidity monolithic capillary exhibited the best performance. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available