Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 62, Issue 1, Pages 32-36Publisher
WILEY
DOI: 10.1002/bab.1232
Keywords
furfural and HMF tolerance; efflux pump
Funding
- A*STAR in Singapore [ICES/12-574A01]
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The presence of the highly toxic furfural and hydroxymethylfurfural (HMF) in the hydrolysate of lignocellulosic biomass prompted the investigation of the Escherichia coli acrR regulatory mutant for higher tolerance to these compounds, to facilitate the production of biofuels and biochemicals, and further biocatalytic conversions. In comparison with the parental strain, the regulatory mutant with the upregulated efflux pump AcrAB-TolC produced moderately better growth and higher tolerance to concentrations of furfural and HMF between 1 and 2gL(-1).
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