Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 60, Issue 5, Pages 527-535Publisher
WILEY
DOI: 10.1002/bab.1121
Keywords
aqueous two-phase system; bromelain; pineapple waste; polyethylene glycol; poly(acrylic acid)
Funding
- Coordination for the Improvement of Higher Level Personnel (Capes)
- National Council for Scientific and Technological Development (CNPq)
- State of Sao Paulo Research Foundation (Fapesp), Brazil
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Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process. (C) 2013 International Union of Biochemistry and Molecular Biology, Inc. Volume 60, Number 5, Pages 527-535, 2013
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