Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 59, Issue 5, Pages 346-352Publisher
WILEY
DOI: 10.1002/bab.1036
Keywords
chitosan-linked PEI; dendritic cells; gene delivery; immunotherapy; nanoparticle; nonviral vector
Funding
- National Natural Science Foundation of China [30973648]
- gNSFC-JSPS joint grant supported by both China and Japan [81011140077]
- Natural Science Foundation of Zhejiang Province, China [R2090176]
- Fundamental Research Funds for the Central Universities
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A dendritic cell (DC) networking system has become an attractive approach in cancer immunotherapy. Successful DC gene engineering depends on the development of transgene vectors. A cationic polymer, chitosan-linked polyethylenimine (PEI) (CP), possessing the advantages of both PEI and chitosan, has been applied in nonviral transfection of DCs. Physicochemical evaluation showed that CP/DNA complexes could form cationic nanoparticles. Compared with DCs transfected with commercial reagent, Lipofectamine2000, it showed higher transfection efficiency and lower cytotoxicity when DCs were transfected with CP/DNA complexes. A nuclear trafficking observation of CP/DNA complexes by a confocal laser scanning microscope further revealed that the CP could help DNA enter into the cytoplasm and finally into the nucleus of a DC. Finally, vaccination of DCs transfected with CP/DNA encoding gp100 slightly improved resistance to the B16BL6 melanoma challenge. This is the first report that CP polymer is used as a nonviral vector for DC gene delivery and DC vaccine. Essentially, these results might be helpful to design a promising nonviral vector for DC gene delivery.
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