Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 57, Issue -, Pages 55-62Publisher
WILEY
DOI: 10.1042/BA20100081
Keywords
carbon/nitrogen molar ratio; fed-batch culture; human-like collagen II; metabolic-flux analysis (MFA); recombinant Escherichia coli
Funding
- National High Technology Research and Development Program of China (863 Program) [2006AA02Z246, 2007AA03Z456]
- National Natural Science Foundation of China [20776119, 21076169]
- Xi'an Research and Development Program [CX0735]
- Shaanxi Provincial Department of Education, China [07JK417, 07JC16, JG08181]
- Natural Science Foundation of Shaanxi Province [2010JQ2012]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20096101120023, 20096101110014]
- Shaanxi Key Subject Program, China
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Recombinant Escherichia coli BL2I was used to produce human-like collagen II in fed-batch cultivation. By performing MFA (metabolic-flux analysis), the carbon/nitrogen molar ratios in both the batch and feeding media were optimized for high-level production of human-like collagen II. Three carbon/nitrogen molar ratios in both the batch and feeding media were used in the present study, and the MFA results showed that the optimal carbon/nitrogen molar ratios for the batch and feeding media were 2.36:1 and 5.12:1 respectively, yielding the highest dry-cell density (67.2 g/l dry cell weight) and human-like collagen production (10.8 g/l).
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