Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1423, Issue -, Pages 79-85Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2015.10.064
Keywords
HPAEC-MS; Dextran; Branches; Qualitative analysis; Quantitative analysis
Funding
- National Natural Science Foundation of China [81473179]
- Jiangsu Specially-Appointed Professor Research Foundation [SR13200113]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [YX13200111]
- funding for Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases [BM2013003]
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Dextran, a family of natural polysaccharides, consists of an alpha (1 -> 46) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method. (C) 2015 Elsevier B.V. All rights reserved.
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