Journal
BIOTECHNOLOGY ADVANCES
Volume 32, Issue 7, Pages 1237-1244Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2014.07.004
Keywords
DNA barcode; Plant authentication; Adverse reactions; Safety; ITS2; psbA-trnH
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Funding
- National Natural Science Foundation of China [81130069, 81373922]
- Fundamental Research Funds for the Central Scientific Research Institutes for Public Welfare [YZ-12-08]
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Numerous adverse reactions have arisen following the use of inaccurately identified medicinal plant ingredients, resulting in conditions such as aristolochic acid nephropathy and herb-induced poisoning. This problem has prompted increased global concern over the safety of herbal medicines. DNA barcoding, a technique aiming at detecting species-specific differences in a short region of DNA, provides a powerful new tool for addressing this problem. A preliminary system for DNA barcoding herbal materials has been established based on a two-locus combination of ITS2 + psbA-trnH barcodes. There are 78,847 sequences belonging to 23,262 species in the system, which include more than 95% of crude herbal drugs in pharmacopeia, such as those of China, Japan, Korea, India, USA, and Europe. The system has been widely used in traditional herbal medicine enterprises. This review summarizes recent key advances in the DNA barcoding of medicinal plant ingredients (herbal materia medica) as a contribution towards safe and efficacious herbal medicines. (C) 2014 Elsevier Inc. All rights reserved.
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