Journal
BIOTECHNOLOGY ADVANCES
Volume 32, Issue 8, Pages 1429-1447Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2014.09.003
Keywords
Hydrogen peroxide; Oxido-reductase; Haem-enzyme; Catalase peroxidase; Purification; Downstream processing; Protein structure
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Catalase, an oxidoreductase enzyme, works as a detoxification system inside living cells against reactive oxygen species formed as a by-product of different metabolic reactions. The enzyme is found in a wide range of aerobic and anaerobic organisms. Catalase has also been employed in various analytical and diagnostic methods in the form of biosensors and biomarkers in addition to its other applications in textile, paper, food and pharmaceutical industries. New applications for catalases are constantly emerging thanks to their high turnover rate, distinct evolutionary origin, relatively simple and well-defined reaction mechanisms. The following review provides comprehensive information on isolation, production and purification of catalases with different techniques from various microbial sources along with their types, structure, mechanism of action and applications. (c) 2014 Elsevier Inc All rights reserved.
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