Journal
BIOTECHNOLOGY ADVANCES
Volume 32, Issue 2, Pages 243-254Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2013.10.006
Keywords
Three-dimensional cell culture; Perfusion culture; Physiological microenvironment; Scaffolds; Tissue engineering; Extracellular matrix
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Funding
- UK Biotechnology and Biological Science Research Council
- Biotechnology and Biological Sciences Research Council [BB/L003961/1] Funding Source: researchfish
- BBSRC [BB/L003961/1] Funding Source: UKRI
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Compelling evidence suggests the limitation and shortcomings of the current and well established cell culture method using multi-well plates, flasks and Petri dishes. These are particularly important when cell functions are sensitive to the local microenvironment, cell-cell and cell-extracellular matrix interactions. There is a clear need for advanced cell culture systems which mimic in vivo and more physiological conditions. This review summarises and analyses recent progress in three dimensional (3D) cell culture with perfusion as the next generation cell culture tools, while excluding engineered tissue culture where three dimensional scaffold has to be used for structural support and perfusion for overcoming mass transfer control. Apart from research activities in academic community, product development in industry is also included in this review. (C) 2013 Elsevier Inc All rights reserved.
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