Lipase immobilized by modification-coupled and adsorption-cross-linking methods: A comparative study

Candida antarctica lipase was immobilized by an adsorption and cross-linking method with NW-ZT2 and by modification-coupled method with a silica-PEG gel. The final product silica-PEG-lipase was confirmed by IR spectra. The optimum pH value, the optimum temperature, the thermo-stabilities and operational stabilities for two kinds of immobilized lipase were also determined. Results show that the silica-PEG-lipase gel was superior to the lipase immobilized by adsorption and cross-linking, however both are viable for use in transesterification reactions. (C) 2010 Elsevier Inc. All rights reserved.