Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1378, Issue -, Pages 1-7Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.11.061
Keywords
Surfactant assisted electromembrane extraction; Nonionic surfactant; Capillary electrophoresis; Basic drugs; Hair; Urine
Funding
- Research Affairs of Shahid Beheshti University
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This paper proposes for the first time the use of a nonionic surfactant for the enhancement of electromembrane extraction performance. The presence of nonionic surfactant in donor phase promotes ionic analytes efficient migration through the organic liquid membrane. Surfactant assisted electromembrane extraction coupled with capillary electrophoresis was used for the extraction of basic drugs (methamphetamine, ephedrine and methadone) from biological samples. Parameters that affect the extraction efficiency were investigated and optimized for the proposed method. Optimal extractions were accomplished with 2-nitrophenyl octyl ether as the supported liquid membrane, with 125 V as the driving force and 0.2 mM Triton X-100 with pH 5.0 in donor and pH 1 in acceptor solutions. Equilibrium extraction conditions were obtained after 20 min of operation where the whole assembly agitated at 1000 rpm. Under the optimum experimental conditions, good limits of detection (0.90-2.42 ng mL(-1)), linearities (R-2 > 0.9995), and repeatability of extraction (RSDs below 4.4%, n = 5) were obtained. Finally, the developed method was applied to drug level monitoring in the biological samples including hair and urine samples and satisfactory results were obtained. (C) 2014 Elsevier B.V. All rights reserved.
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