Journal
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
Volume 24, Issue 2, Pages 1854-1857Publisher
TAYLOR & FRANCIS LTD
DOI: 10.2478/V10133-010-0028-0
Keywords
lycopene; enzymes; tomato paste; cell-wall polysaccharides; bioprocessing
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Four commercial enzyme preparations containing cell-wall degrading enzyme activities were tested for their ability to facilitate lycopene extraction from tomato paste. At 25 degrees C, up to 75.6% of the lycopene present in the tomato product was extracted by a two-stage procedure that consisted of a preliminary 5 hour enzyme incubation followed by a 3 hour solvent (hexane or ethyl acetate) extraction. Increasing the duration of the enzymatic treatment to 12-18 h allowed the recovery of 85-90% of total lycopene. The highest extraction yields were achieved by using enzyme preparations' with polygalacturonase and pectin methylesterase in addition to pectin lyase or cellulase activities.
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