Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1425, Issue -, Pages 213-220Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2015.11.044
Keywords
N-glycopeptides enrichment; Magnetic nanoparticles; Polyethyleneimine; Click chemistry; Hydrophilic interaction chromatography
Funding
- China State Key Basic Research Program [2013CB911203, 2012CB910601]
- Creative Research Group by NSFC [21321064]
- National Natural Science Foundation of China [21235006, 21535008, 21305139]
- Youth Innovation Promotion Association CAS [2014164]
Ask authors/readers for more resources
Hydrophilic interaction chromatography (HILIC) adsorbents have drawn increasing attention in recent years due to their high efficiency in N-glycopeptides enrichment. The hydrophilicity and binding capacity of HILIC adsorbents are crucial to the enrichment efficiency and mass spectrometry (MS) detection sensitivity of N-glycopeptides. Herein, magnetic nanoparticles coated with maltose-functionalized polyethyleneimine (Fe3O4-PEI-Maltose MNPs) were prepared by one-pot solvothermal reaction coupled with click chemistry and utilized for N-glycopeptides enrichment. Owing to the presence of hydrophilic and branched polyethyleneimine, the amount of immobilized disaccharide units was improved about four times. The N-glycopeptides capturing capacity was about 150 mg/g (IgG/MNPs) and the MS detection limitation as low as 115 fmol for IgG and 85% average enrichment recovery were feasibly achieved by using this hybrid magnetic adsorbent. Finally, 1237 unique N-glycosylation sites and 1567 unique N-glycopeptides from 684 N-glycoproteins were reliably characterized from 60 mu g protein sample extracted from mouse liver. Therefore, this maltose-functionalized polyethyleneimine coated adsorbent can play a promising role in highly efficient N-glycopeptides enrichment for glycoproteomic analyses of complex protein samples. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available